Official website of gene sequence digital currency crowdfunding

1. "Coin profit China" digital asset trading platform is committed to the practical application of blockchain technology. Digital design of various assets such as equity and usufruct assets, debt assets, proct crowdfunding, and consumer crowdfunding, as well as subsequent value-added and value realization, are the focus and research fields of "coin profit China", First of all, the platform focuses on the vast number of small and micro enterprises and entrepreneurial groups as well as the emerging "middle class" in the "general" word, and makes full use of the principle of equality among the participating nodes in the blockchain technology in the "Hui" word, so as to achieve the goal of public governance and sharing, completely change the current business chaos of "too much information, too little trust", and reconstruct the business trust system with blockchain technology.

2. Want to buy the first flush number?

3. The most reliable thing is to look up the relevant literature about the genes you want to do, which all have the gene registration number; If it's not the organism you're going to do, you can also find out the genes in the literature, and then blast them to find the corresponding sequences in the organism you're doing.

4. First of all, your website is not right, https://www.ncbi.nlm.nih.gov/genbank/ Second, select the database, enter your gene number, search, and download.

5. How To: Submit data to NCBI
http://www.ncbi.nlm.nih.gov/guide/howto/submit-sequence-data/
here is a detailed introction. Need a small tool in NCBI, there are instructions for their own reference, English can understand no problem. If you don't understand, ask me again.

6. The release time of these three versions is different. In addition, they are published by different organizations
grch37.p2 was published by genome reference consortium in July 2010,
huref was published by J Craig Venter Institute in May 2007,
Celera was published by Celera company in November 2001,
the sequences of these three versions are different, but they are all gene sequences of human chromosome 1, All of them are available for reference. Grch37.p2 is the latest and perhaps the most detailed version< br />
Each file is named according to the abbreviation for the species,
whether the assembly is the reference assembly (_ ref_) or an alternate
assembly (_ alt_), The assembly name, and either the chrome label
or the scaffold group (nonlocalized, unplaced, or alts)_ ref_ Represents the reference assembly sequence or_ alt_ Represents an alternative assembly sequence; Assembly sequence name; And the chromosome number or frame group (nonlocalized, unplaced, or alts)

7. There is blast on the home page. Click in to select the corresponding database (human or mouse source, nucleic acid sequence or protein sequence). Click in and input the sequence blast

8. Find the gene sequence above NCBI. The method is: first open the NCBI website home page, then select nucleoside in the search column, enter the name of the gene sequence in the box, and click search. A lot of results will come out, because many genes have the same name, or a gene is different in different species. Just look for the gene sequence you want. Note that to determine whether the gene name is uniform, it is easy to find the gene sequence and protein sequence, because the result will show the protein sequence of the gene. You can select protein at the beginning of the search and find the protein sequence.

9. Luciferase reporter gene system is a kind of reporter system which uses luciferase as substrate to detect luciferase activity of firefly
luciferase can catalyze the oxidation of luciferin to oxyluciferin, which will emit bioluminescence. Then, the bioluminescence released ring the oxidation process of luciferin can be determined by fluorescence analyzer, also known as chemiluminescence analyzer or liquid scintillation analyzer.