Ore distribution in cold crystal pool

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Generally speaking, large infectious disease hospitals will have this test. Then there are some provincial centers for Disease Control and prevention, or some regional third-party testing institutions, which can carry out testing

the substance detected by nucleic acid is the nucleic acid of virus. Nucleic acid detection is to find the presence of viruses in the respiratory tract, blood or feces of patients, to determine whether they are infected by COVID-19. Therefore, once the detection of nucleic acid "positive", it can prove that there is a virus in the patient's body

after COVID-19 infects the human body, it will first propagate in the respiratory tract, so it can detect whether the human body is infected with the virus by detecting the nucleic acid in the sputum and nasopharyngeal swabs. Therefore, novel coronavirus positive test can be used as the gold standard for the diagnosis of new coronavirus infection. P>

extended data: the most common method of

detection of New Coronavirus specific sequence is fluorescence quantitative PCR (polymerase chain reaction). Because the PCR reaction template is only DNA, the New Coronavirus nucleic acid (RNA) should be RRT to DNA before the PCR reaction. p>

in the PCR reaction system, a pair of specific primers and a TaqMan probe were included. The probe was a specific oligonucleotide sequence with reporter fluorescent group and quenching fluorescent group labeled at both ends. When the probe is intact, the fluorescence signal emitted by the reporting group is absorbed by the quenching group

if there is a target sequence in the reaction system, the probe will bind to the template ring PCR reaction, the DNA polymerase will cleave the probe along the template by using the exonuclease activity of the enzyme, and the reporter group and quenching group will be separated to emit fluorescence

for each DNA strand amplified, a fluorescent molecule was proced. The fluorescence quantitative PCR instrument can monitor that the number of cycles (CT value) of fluorescence reaching the preset threshold is related to the concentration of viral nucleic acid. The higher the concentration of viral nucleic acid is, the smaller the CT value is. The procts of different manufacturers will determine the positive judgment value of the proct according to the performance of their own procts